Figure Preparation

Prepare figures with applications capable of creating high-resolution TIFF or EPS files. Industry-standard applications such as Illustrator (for EPS) or Photoshop (for TIFF) offer the tools to produce suitable images.

Rapid Inspector - FREE!

Rapid Inspector allows authors to check figure files for format, resolution, color space and other requirements.

Download the FREE Rapid Inspector application here.


Always maintain unedited source materials for all images as captured before any editing required for submission.


The Journal of Biological Chemistry maintains strict standards for acceptance of digital images. The author is responsible for verifying that graphics are suitable for scientific review. In all cases, the final format for digital art submission must be EPS or TIFF files.

To avoid inaccurate presentation of the data, resolution of all submitted images must be 300 dpi. For optimal reproduction online, color figures must be in the RGB color mode.

Multipanel figures (figures with parts labeled a, b, c, d, etc.) should be assembled into a composite - that is, the multipanel elements should not be separated and submitted as different files. Numbers, letters, and symbols used throughout a multipanel figure must be consistent in font size and style. The smallest font size in a figure must not be less than 70% of the largest font in that figure. For more details about preparing digital art, including multipanel figures, see "Guidelines" (above).

To submit figures for an accepted JBC manuscript, click RAPID SUBMISSION.


Image Manipulation

While editing of images is often needed for clarity and/or brevity, the manipulation for deceptive purposes, or to unfairly enhance, eliminate, or obscure data, is misconduct and will be addressed as such.

For graphic material, The Journal of Biological Chemistry has adopted the following policy of the Journal of Cell Biology:

"No specific feature within an image may be enhanced, obscured, moved, removed, or introduced. The groupings of images from different parts of the same gel, or from different gels, fields or exposures must be made explicit by the arrangement of the figure (e.g. using dividing lines) and in the text of the figure legend. Adjustments of brightness, contrast, or color balance are acceptable if they are applied to every pixel in the image and as long as they do not obscure, eliminate, or misrepresent any information present in the original, including the background. Nonlinear adjustments (e.g. changes to gamma settings) must be disclosed in the figure legend."